HaloTag: a new versatile reporter gene system in plant cells.
Identifieur interne : 003E00 ( Main/Exploration ); précédent : 003D99; suivant : 003E01HaloTag: a new versatile reporter gene system in plant cells.
Auteurs : Christina Lang [Allemagne] ; Jutta Schulze ; Ralf-R Mendel ; Robert H NschSource :
- Journal of experimental botany [ 0022-0957 ] ; 2006.
Descripteurs français
- KwdFr :
- Cellules cultivées (MeSH), Gènes rapporteurs (MeSH), Ingénierie des protéines (méthodes), Microscopie confocale (MeSH), Microscopie de fluorescence (MeSH), Paroi cellulaire (métabolisme), Populus (cytologie), Populus (génétique), Populus (métabolisme), Protoplastes (cytologie), Protoplastes (métabolisme), Protéines luminescentes (analyse), Tabac (cytologie), Tabac (génétique), Tabac (métabolisme), Vecteurs génétiques (MeSH).
- MESH :
- analyse : Protéines luminescentes.
- cytologie : Populus, Protoplastes, Tabac.
- génétique : Populus, Tabac.
- métabolisme : Paroi cellulaire, Populus, Protoplastes, Tabac.
- méthodes : Ingénierie des protéines.
- Cellules cultivées, Gènes rapporteurs, Microscopie confocale, Microscopie de fluorescence, Vecteurs génétiques.
English descriptors
- KwdEn :
- Cell Wall (metabolism), Cells, Cultured (MeSH), Genes, Reporter (MeSH), Genetic Vectors (MeSH), Luminescent Proteins (analysis), Microscopy, Confocal (MeSH), Microscopy, Fluorescence (MeSH), Populus (cytology), Populus (genetics), Populus (metabolism), Protein Engineering (methods), Protoplasts (cytology), Protoplasts (metabolism), Tobacco (cytology), Tobacco (genetics), Tobacco (metabolism).
- MESH :
- chemical , analysis : Luminescent Proteins.
- cytology : Populus, Protoplasts, Tobacco.
- genetics : Populus, Tobacco.
- metabolism : Cell Wall, Populus, Protoplasts, Tobacco.
- methods : Protein Engineering.
- Cells, Cultured, Genes, Reporter, Genetic Vectors, Microscopy, Confocal, Microscopy, Fluorescence.
Abstract
HaloTag Interchangeable Labeling Technology (HaloTag) was originally developed for mammalian cell analysis. In this report, the use of HaloTag is demonstrated in plant cells for the first time. This system allows different fluorescent colours to be used to visualize the localization of the non-fluorescent HaloTag protein within living cells. A vector was constructed which expresses the HaloTag protein under the control of the 35S promoter of cauliflower mosaic virus. The functionality of the HaloTag construct was tested in transient assays by (i) transforming tobacco protoplasts and (ii) using biolistic transformation of intact leaf cells of tobacco and poplar plants. Two to fourteen days after transformation, the plant material was incubated with ligands specific for labelling the HaloTag protein, and fluorescence was visualized by confocal laser scanning microscopy. The results demonstrate that HaloTag technology is a flexible system which generates efficient fluorescence in different types of plant cells. The ligand-specific labelling of HaloTag protein was not hampered by the plant cell wall.
DOI: 10.1093/jxb/erl065
PubMed: 16873446
Affiliations:
Links toward previous steps (curation, corpus...)
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sort="Mendel, Ralf R" uniqKey="Mendel R" first="Ralf-R" last="Mendel">Ralf-R Mendel</name></author><author><name sortKey="H Nsch, Robert" sort="H Nsch, Robert" uniqKey="H Nsch R" first="Robert" last="H Nsch">Robert H Nsch</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16873446</idno><idno type="pmid">16873446</idno><idno type="doi">10.1093/jxb/erl065</idno><idno type="wicri:Area/Main/Corpus">003D63</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003D63</idno><idno type="wicri:Area/Main/Curation">003D63</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003D63</idno><idno type="wicri:Area/Main/Exploration">003D63</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">HaloTag: a new versatile reporter gene system in plant cells.</title><author><name sortKey="Lang, Christina" sort="Lang, Christina" uniqKey="Lang C" first="Christina" last="Lang">Christina Lang</name><affiliation wicri:level="1"><nlm:affiliation>Institut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstrasse 1, D-38106 Braunschweig, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstrasse 1, D-38106 Braunschweig</wicri:regionArea><wicri:noRegion>38106 Braunschweig</wicri:noRegion><wicri:noRegion>38106 Braunschweig</wicri:noRegion><wicri:noRegion>D-38106 Braunschweig</wicri:noRegion></affiliation></author><author><name sortKey="Schulze, Jutta" sort="Schulze, Jutta" uniqKey="Schulze J" first="Jutta" last="Schulze">Jutta Schulze</name></author><author><name sortKey="Mendel, Ralf R" sort="Mendel, Ralf R" uniqKey="Mendel R" first="Ralf-R" last="Mendel">Ralf-R Mendel</name></author><author><name sortKey="H Nsch, Robert" sort="H Nsch, Robert" uniqKey="H Nsch R" first="Robert" last="H Nsch">Robert H Nsch</name></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="ISSN">0022-0957</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (metabolism)</term><term>Cells, Cultured (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Genetic Vectors (MeSH)</term><term>Luminescent Proteins (analysis)</term><term>Microscopy, Confocal (MeSH)</term><term>Microscopy, Fluorescence (MeSH)</term><term>Populus (cytology)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Protein Engineering (methods)</term><term>Protoplasts (cytology)</term><term>Protoplasts (metabolism)</term><term>Tobacco (cytology)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules cultivées (MeSH)</term><term>Gènes rapporteurs (MeSH)</term><term>Ingénierie des protéines (méthodes)</term><term>Microscopie confocale (MeSH)</term><term>Microscopie de fluorescence (MeSH)</term><term>Paroi cellulaire (métabolisme)</term><term>Populus (cytologie)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protoplastes (cytologie)</term><term>Protoplastes (métabolisme)</term><term>Protéines luminescentes (analyse)</term><term>Tabac (cytologie)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Vecteurs génétiques (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Luminescent Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines luminescentes</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Populus</term><term>Protoplastes</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Populus</term><term>Protoplasts</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Populus</term><term>Protoplasts</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Protein Engineering</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Paroi cellulaire</term><term>Populus</term><term>Protoplastes</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Ingénierie des protéines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>Genes, Reporter</term><term>Genetic Vectors</term><term>Microscopy, Confocal</term><term>Microscopy, Fluorescence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Gènes rapporteurs</term><term>Microscopie confocale</term><term>Microscopie de fluorescence</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">HaloTag Interchangeable Labeling Technology (HaloTag) was originally developed for mammalian cell analysis. In this report, the use of HaloTag is demonstrated in plant cells for the first time. This system allows different fluorescent colours to be used to visualize the localization of the non-fluorescent HaloTag protein within living cells. A vector was constructed which expresses the HaloTag protein under the control of the 35S promoter of cauliflower mosaic virus. The functionality of the HaloTag construct was tested in transient assays by (i) transforming tobacco protoplasts and (ii) using biolistic transformation of intact leaf cells of tobacco and poplar plants. Two to fourteen days after transformation, the plant material was incubated with ligands specific for labelling the HaloTag protein, and fluorescence was visualized by confocal laser scanning microscopy. The results demonstrate that HaloTag technology is a flexible system which generates efficient fluorescence in different types of plant cells. The ligand-specific labelling of HaloTag protein was not hampered by the plant cell wall.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16873446</PMID><DateCompleted><Year>2006</Year><Month>12</Month><Day>08</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0022-0957</ISSN><JournalIssue CitedMedium="Print"><Volume>57</Volume><Issue>12</Issue><PubDate><Year>2006</Year></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>HaloTag: a new versatile reporter gene system in plant cells.</ArticleTitle><Pagination><MedlinePgn>2985-92</MedlinePgn></Pagination><Abstract><AbstractText>HaloTag Interchangeable Labeling Technology (HaloTag) was originally developed for mammalian cell analysis. In this report, the use of HaloTag is demonstrated in plant cells for the first time. This system allows different fluorescent colours to be used to visualize the localization of the non-fluorescent HaloTag protein within living cells. A vector was constructed which expresses the HaloTag protein under the control of the 35S promoter of cauliflower mosaic virus. The functionality of the HaloTag construct was tested in transient assays by (i) transforming tobacco protoplasts and (ii) using biolistic transformation of intact leaf cells of tobacco and poplar plants. Two to fourteen days after transformation, the plant material was incubated with ligands specific for labelling the HaloTag protein, and fluorescence was visualized by confocal laser scanning microscopy. The results demonstrate that HaloTag technology is a flexible system which generates efficient fluorescence in different types of plant cells. The ligand-specific labelling of HaloTag protein was not hampered by the plant cell wall.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lang</LastName><ForeName>Christina</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Institut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstrasse 1, D-38106 Braunschweig, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schulze</LastName><ForeName>Jutta</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Mendel</LastName><ForeName>Ralf-R</ForeName><Initials>RR</Initials></Author><Author ValidYN="Y"><LastName>Hänsch</LastName><ForeName>Robert</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>07</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008164">Luminescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="Y">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008164" MajorTopicYN="N">Luminescent Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008856" MajorTopicYN="Y">Microscopy, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015202" MajorTopicYN="N">Protein Engineering</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011523" MajorTopicYN="N">Protoplasts</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>7</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>12</Month><Day>12</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>7</Month><Day>29</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16873446</ArticleId><ArticleId IdType="pii">erl065</ArticleId><ArticleId IdType="doi">10.1093/jxb/erl065</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="H Nsch, Robert" sort="H Nsch, Robert" uniqKey="H Nsch R" first="Robert" last="H Nsch">Robert H Nsch</name><name sortKey="Mendel, Ralf R" sort="Mendel, Ralf R" uniqKey="Mendel R" first="Ralf-R" last="Mendel">Ralf-R Mendel</name><name sortKey="Schulze, Jutta" sort="Schulze, Jutta" uniqKey="Schulze J" first="Jutta" last="Schulze">Jutta Schulze</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Lang, Christina" sort="Lang, Christina" uniqKey="Lang C" first="Christina" last="Lang">Christina Lang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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